Donna Murphy (born March 7, 1959) is an American actress and singer, known for her work in musical theater. A five-time Tony Award nominee, she has twice won the Tony for Best Actress in a Musical: for her role as Fosca in Passion (1994–95) and as Anna Leonowens in The King and I (1996–97). She was also nominated for her roles as Ruth Sherwood in Wonderful Town (2003), Lotte Lenya in LoveMusik (2007) and Bubbie/Raisel in The People in the Picture (2011).

Murphy made her Broadway debut as a replacement in the 1979 musical They're Playing Our Song. Her other stage credits include the original Off-Broadway productions of Song of Singapore (1991) and Hello Again (1993). In 1997, she won a Daytime Emmy Award for Outstanding Performer in a Children's Special for her role in Someone Had to be Benny, an episode of the HBO series Lifestories: Families in Crisis. Her film roles include Anij in Star Trek: Insurrection (1998); Rosalie Octavius in Spider-Man 2 (2004); Mother Gothel in the animated film Tangled (2010), and one of the government secretaries in The Bourne Legacy (2012).